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Poon et al., 2004

Plasmids construction The genes of TFR1, NCOA4, and FTH1 were amplified from PK-15 cells and then ligated into pCMV-HA vectors (Clontech, 631604) and p3FLAG-CMV-10 vectors (Sigma, E4401) with the use of DNA assembly mix plus (D0204P, Lablead)

Patients with PCOS display characteristic gut microbiota dysbiosis, though specific compositional alterations remain inconsistently reported across studies (36, 37)