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The concentration of the extracted double-stranded DNA was determined using an Infinite M200 PRO microplate reader (Tecan Austria GmbH, Grdig, Austria)

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In line with this, recent findings indicate that oral microbiome dysbiosis, particularly a reduction in health associated genera such as Leptotrichia , may contribute to pregnancy loss by impairing metabolic pathways (like L-arginine degradation) critical for vascular health and placental function 59