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The harmful events were on the left colic artery and vein, such as two ligations (IC rats) or injuries from removed ligations (RL) (IC + RL rats) (there was always gross vessel presentation failure), and the combination of two obstructions, ligation of the vessels and additional colon obstruction (OB) (IC + OB rats)

This is the practical reason a reader who is nervous about small-volume accuracy might prefer the 50 mg protocol the trade-off being that each vial carries fewer total doses, as the supply math shows next

doi: 10.1042/bj3440109