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Several theories have been proposed to explain the mechanism underlying ovarian aging, including free radical theory, apoptosis, telomere shortening, mitochondrial dysfunction, and inflammation theory (Shi et al., 2016

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Precautions involve avoiding direct eye contact and discontinuing if severe irritation occurs

On the following morning, a fresh bacterial culture (OD 600 = 0.5 in M9 medium) was prepared as described above