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One individual per group was euthanized before addressing the next animal in each group to render differences arising from the time of day at euthanization (e.g., cecal mass changes resulting from differences in time post-food consumption) comparable across all groups

High doses may cause temporary headaches due to increased NAD+ turnover and mitochondrial activity

Moreover, the magnitude of this effect significantly depended on the dosage: median time to death, or ventilation support, was 570 days for placebo, 1,087 days for 25 mg, and 1,197 days for 50 mg methylcobalamin groups

These observations help identify the actual source