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A Couple Things That May Cause You Concern Occasionally I receive calls from parents concerned about a bluish or reddish discoloration under the skin and they are worried that this is a bad thing, that they have hurt their child, or that the dark spot will be permanent

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Transfection was carried out using Lipofectamine 3000 Kit on cells at 50% confluence for 6 hours, followed by protein extraction 48 hours post-transfection