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After that, nucleus were stained with PhyLight Enhanced Antifade Mounting Medium with DAPI (Phygene, China) for 5 min

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Animal studies and a very small uncontrolled human pilot study exist

This is the key observation underlying the importance ascribed to the close redox coupling of ascorbate and glutathione pools in vivo , which allows glutathione to play an indirect role in H 2 O 2 reduction as part of a reaction sequence that ultimately depends on electrons derived from NAD(P)H and/or ferredoxin (Figure 1)

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