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A recent investigation of its mechanism of action showed that AcM does not exhibit direct antimicrobial activity as it does not target the PGN of live bacteria but rather luminal cell-free PGN fragments, whilst it is able to promote anti-inflammatory pathways through nucleotide-binding oligomerization domain-containing protein 2 receptor activation [24]

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This is not a subtle effect

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Then, each of six redox metabolites is measured in cyclic enzymatic assay system, followed by colorimetric analysis