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Ghrelin in fetal thyroid and follicular tumors and cell lines expression and effects on tumor growth

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Metabolome analysis Approximately 50 mg of frozen tissue was plunged into 1500 L of 50% acetonitrile/Milli-Q water containing internal standards (Solution ID: 304-1002, Human Metabolome Technologies, Inc., Tsuruoka, Japan) at 0 C in order to inactivate the enzymes present in the cells