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However, significant GSSG accumulation is also observed in gr1 knockout mutants, which are aphenotypic (Marty et al., 2009

For cell samples, cells were lysed with lysis buffer (50 mM Tris-HCl [pH 8.0], 1% Triton X-100, 0.5% Nonidet P-40, 10 mM dithiothreitol, 1% protease inhibitor cocktail, 150 mM NaCl, and 5 mM EDTA) on ice for 30 min, followed by centrifugation (12,000 g , 20 min, and 4 C)

[85] demonstrated the analgesic effects and substantial decrease in abdominal writhing of P

A 5mg vial lasts 17-50 days