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Caspase-3 is involved in the activation cascade in apoptosis, so increased caspase-3 activity can act as a marker for DNA fragmentation and apoptosis in injured and degenerated disc tissue 33

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--- Potential Risks and Considerations Because BPC-157 and TB-500 are not approved veterinary drugs, potential risks may include: Unknown long-term safety effects Variability in product quality or sterility Contamination risks with unregulated suppliers Potential interactions with other medications or supplements Owner supervision alone is not a substitute for veterinary guidance

To assess the G/D-CuPs biocompatibility and its ability to support cell growth, we performed in vitro three-dimensional (3D) culture experiments using NIH/3T3 cells as a model