Measurement of the levels of intracellular GSH and the relative expression of NRF2, GPX4 and SOD1 in tissue samples following neoadjuvant chemotherapy To further verify the role of GSH metabolism in chemotherapy resistance, we analyzed breast cancer data from the Gene Expression Omnibus (GEO) dataset (GEO: GSE140494)
Its induction exerts direct tumor-suppressive effects through iron-mediated oxidative damage, yet paradoxically drives pro-tumorigenic adaptations in the tumor microenvironment (TME), particularly in advanced PCa with dysregulated iron metabolism [66,67]
The slides were treated with a lysis solution (2.5 M NaCl, 100 mM EDTA, 100 mM TRIS, 1% Triton X-100, 10% DMSO, pH ~ 10.2) for 24 h at 4C
Most patients require 810 sessions (weekly or bi-weekly), followed by maintenance
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