Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen
When administered into the brain circulation and cerebrospinal fluid of mice, DSIP showed a significant, dose-dependent reduction in pain response in the tail-pinch and hot-plate tests
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EMT is observed in the process of renal interstitial, pulmonary and liver fibrosis [56,57,58,59], as well as in specific ocular tissue [32]