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The activation of GPER has been reported to stimulate the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway [121, 122], resulting in the upregulation of the expression of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GPx) [123]

Proc Am Assoc Cancer Res 46:1254, 2005

Total cell lysates were prepared using lysis buffer (20 mM Hepes (pH 7.4), 150 mM NaCl, 50 mM KF, 50 mM -glycerolphosphate, 2 mM EGTA (pH 8), 1 mM Na 3 VO 4 , 1% Triton X-100, 10% glycerol and 1 protease inhibitor cocktail (Calbiochem))

110 However, treatment with A-485 lowers H3K18la levels and suppresses osteogenic gene expression in BMSCs, thereby exacerbating osteoporosis

Koshelskaya, N