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The key is doing it safely, with the right medications and the right team behind you

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I make sure I move away from my desk at least every hour (usually to visit the toilet after all that water)

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Once the lysate had been loaded, the column was washed with 20 column volumes (CV) wash buffer, and the His-tagged TRFH domain was eluted using a linear gradient from 0 to 100% elution buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 1 mM TCEP, 300 mM imidazole) over 25 CV