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We monitored these FA parameters by immunolabelling U-2 OS cells for the FA marker paxillin 3 followed by automated confocal microscopy and image analysis (Fig

10.1186/s12943-022-01587-9 66 GuX.MuC.ZhengR.ZhangZ.ZhangQ.LiangT

Total NO (nitrite and nitrate) levels in the serum and follicular fluid assay of NO are measured via a rapid-response chemiluminescence analyzer [29]

GSH is a critically important molecule in both direct reactions with oxidants as well as several enzymatic detoxification pathways

Free Cu2+ is toxic (Fenton reactions, hydroxyl radicals), but the GHK-Cu complex is stable, diffusible and delivers copper in a targeted manner to copper-dependent metalloenzymes: lysyl oxidase (collagen/elastin crosslinking), Cu/Zn superoxide dismutase (SOD1, antioxidant defence), tyrosinase (controlled melanogenesis), dopamine-beta-hydroxylase