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The comparative threshold cycle (Ct) method was used to measure the relative expression, where 2 Ct represents the fold change in expression, as previously described (Livak and Schmittgen, 2001)

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Through this network-oriented reevaluation, we aim to provide a unifying theoretical perspective that organizes fragmented observations, highlights key regulatory hubs, and generates testable predictions to guide future basic research and the development of potential clinical intervention strategies

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