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Cryo-EM sample preparation and data collection Sucrose gradient purified fractions of the PV3-SC8 or wt PV2 VLPs expressed in yeast and mammalian cells, respectively, were pooled, buffer exchanged into DPBS-EDTA using Zeba Spin Desalting Columns (Thermo Fisher Scientific) with a 7 K molecular weight cut-off (MWCO) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO, Merck Millipore) to a concentration of 0.91.5 mg/ml

3.2.2 Non-dopaminergic Drugs to Treat Motor Fluctuations The 2 pivotal trials of ER amantadine to treat LIDs (see Sect

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These injections combine vitamin B12 with lipotropic agents to provide a synergistic effect that could aid in the weight loss journey of individuals seeking additional support beyond conventional diet and exercise