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doi: 10.1073/pnas.1716173114 39 MoroTEbertSMAdamsCMRasmussenBB

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Novelty-suppressed feeding The novelty-suppressed feeding (NSF) test evaluates the motivation and anxiety levels of mice when exposed to a novel environment following a 24-h fasting period [24]

Western blot and antibodies The equal amounts (30 g) of protein lysed from aortic tissue and HUVECs, were separated by SDS-PAGE and then transferred onto polyvinylidene fluoride (PVDF) membranes (Merck Millipore, IPVH00010)