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Samples are then rinsed 2 times in PBS then incubated in DAPI (1:3000) in PBS for 5 minutes prior to mounting on slides

Our recent study found that NADPH promotes recruitment of ferroptosis suppressor protein 1 (FSP1) to the plasma membrane through N-myristoyltransferase 2 (NMT2), which plays a vital role in resisting KA-mediated neuronal ferroptosis

Locasale JW, Grassian AR, Melman T, Lyssiotis CA, Mattaini KR, Bass AJ, Heffron G, Metallo CM, Muranen T, Sharfi H, Sasaki AT, Anastasiou D, Mullarky E, Vokes NI, Sasaki M, Beroukhim R, Stephanopoulos G, Ligon AH, Meyerson M, Richardson AL, Chin L, Wagner G, Asara JM, Brugge JS, Cantley LC, Vander Heiden MG

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This ultimately leads to harmful lipid peroxide formation (Wang et al., 2021c)