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The most common buffers used to regenerate glutathione beads are alternating 10 column volume washes of: Repeat for a total of three washes with each buffer

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The phenylarsenoxide group is the active moiety, imparting to GSAO its activity by crosslinking closely spaced protein thiols and forming a high affinity ring structure between its arsenic and the thiols ( FIGURE 3 4-( N -( S -glutathionylacetyl)amino)phenylarsonous acid in its original form is essentially membrane impermeable