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The specific antisense sequences were 5-GGCUCCUACACUUCUGUUATT-3 for FoxO4 siRNA and 5-UAACAGAAGUGUAGGAGCCTT-3 for the negative control

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Therefore, WECSL treatment altered the composition of the gut microbiota, especially Lactobacillaceae and Ruminococcaceae , and further promoted the metabolism of WECSL, improving constipation symptoms