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This can be easily explained by the fact that NAC is often used concurrently with pro-oxidant agents and can reduce oxidative stress, most probably by reacting directly with them in the cell culture medium, and even when a pretreatment is declared, in most cases NAC is not washed away before the oxidative insult

Second, integration of single-cell sequencing data provides key cellular context for this mechanism

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Alternatively, a maintenance dose of 1 mg per day taken intermittently throughout the year has also been suggested in longevity communities

PMID 38084426