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Furthermore, environmental pollutants, determining the formation of DNA adducts and abasic sites construction, can induce DNA modifications in gametes and embryos by introducing genetic mutations once unrepaired ( Nutritional Disorders A normal reproductive performance definitely requires a healthy nutrition since malnourished males and females are reported as major infertile populations in developing countries while, eating in excess, fast food consumption, hypercaloric dietary regimens and obesity, concur to infertility in well-developed and western societies

aqueous normal phase (ANP) HPLC, utilising columns like Cogent Diamond Hydride and a mobile phase of deionised water/acetonitrile/0.1% formic acid, offers a rapid, reliable, and sensitive method for EGT quantification and purification [3,35]

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