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Database searching was performed in Proteome Discoverer (PD, Thermo Fisher Scientific) v3.0 against the UniProt human reference proteome, allowing tryptic peptides (730 aa), up to two missed cleavages, and up to three variable modifications per peptide

doi: 10.1177/09603271211021476 84 JoshuaDBKyung-DonKNeilVKHannahEOSamGStacieKTet al

doi: 10.1186/s12967-020-02269-0 32 BedreeHSunnquistMJasonLA

Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid

The low expression of PEBP1 can affect ccRCC progression by affecting tumor angiogenesis by activating the extracellular regulated protein kinase (ERK) signaling pathway[58]