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"Delta sleep-inducing peptide and glucocorticoid-induced leucine zipper: potential links between circadian mechanisms and obesity?"
Research examines its signaling effects without significant impact on cortisol or prolactin
Cells were suspended in 800 L of buffer A (10 mmol/L HEPES-KOH with 1.5 mmol/L MgCl 2 , 10 mmol/L KCl, 0.2 mmol/L phenylmethylsulfonyl fluoride, and 5 mol/L DTT) containing protease inhibitors (5 g/mL each of pepstatin, leupeptin, and aprotinin), and homogenized in a 10-mL Wheaton homogenizer
Individuals sensitive to caffeine