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2 × 105 3
5´ m7GpppN—OH 3´)
PCR was performed according to the manufacturer’s recommendations using primers specific for the indicated genes
Use in the outgrowth stage of competent cell transformation to achieve 10-folder higher transformation efficiency than you would get with LB or SOC media
Modified RNA containing pyrimidine-2´-fluoro-dNMPs
E. cloni-10G ELITE Electrocompetent Cells (SixPacks) E. cloni 10G Chemically Competent Cells 2 × 105 3E. cloni 10G and 10GF' Electrocompetent Cells Direct replacements for standard cloning strains (e. g., DH5, DH10B, JM109, TOP10, XL1 Blue, etc.) Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue white screening capable. Available in a range of high transformation efficiencies (5 109 to 4 1010 cfu g). Convenient packaging options. Lucigens E. cloni competent cells share the most useful genetic elements
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