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Notably, although the NIR absorption peaks of the coupled phage-ICG complexes (PIs) and free ICG were similar, the modified PIs had a broader absorption spectrum, which improved photothermal conversion efficiency ( Figure 3J )

(Redrawn from van der Rest M, Garrone A: Collagen family of proteins

& Zoumpourlis, V

The correct technique: Draw the calculated volume of sterile solvent into a syringe Insert the needle through the rubber stopper at an angle Direct the solvent stream against the inner vial wall, allowing it to run down slowly Remove the needle once all solvent is added Allow the vial to sit undisturbed for several minutes for passive dissolution Gentle Mixing Protocol After solvent addition, gentle swirling promotes complete dissolution without damaging peptide structure

You wouldn't just blast it with a firehose, right