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In an ice water bath, 30L of blood sample was placed in a 96 deep-well plate, 100 L of ultrapure water was added, 500 L of precipitant (methanol) was added, the plate was sealed, vortexed and shaken for 10 min, and centrifuged (4000 rpm) for 15 min

1,8 Cysteine occurs in plasma in the following forms: the solitary amino acid with its free thiol group (free cysteine), a disulfide between 2 molecules of cysteine (cystine), and a mixed disulfide with cysteinyl residues of albumin or other plasma proteins (protein-bound cysteine)

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Local uptake and synthesis of oestrone in normal and malignant postmenopausal breast tissues