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The membrane was further washed with TBST for 4 5 min and Tris-buffered saline without Tween for 2 5 min before incubation with either SuperSignal West Pico PLUS, Femto or Atto chemiluminescent substrate (Thermo Scientific, 34579, 34094 and 38554 to optimize the intensity of the signal

Dixon L, Duncan D, Johnson P, et al

Decreased cAMP results in reduced activation of PKA, a major kinase regulating the activity of hormone-sensitive lipase, HSL

Front Toxicol 2022, 4, 856614 10.3389/ftox.2022.856614

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