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Multi-photon and second harmonic generation microscopy The collagen gels were fixed with 4% paraformaldehyde for 20 minutes and washed in PBS with 0.1% saponin and 0.1% Tween 20 before being incubated with phalloidin conjugated with Alexa Fluor 594 for 1 h at room temp

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Temperature-induced degradation does not just reduce potency

A common approach is topical GHK-Cu at night and minoxidil in the morning to avoid pH and formulation conflicts, with oral or topical finasteride added for DHT-driven loss

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