After incubation in a 100% ethanolchloroform (1:1) mixture for 30 min and in chloroform (I) for 1 h, the specimens were left overnight in chloroform (II) and the tissues were then impregnated with paraffin (3 changes, 1 h each) at 60C
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This includes vegans and vegetarians, older adults, individuals with atrophic gastritis, pernicious anemia, gastrointestinal disorders, those who have had gastrointestinal surgery and people who take certain medications including, but not limited to, estrogen (HRT), and birth control pills