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We originally used acyclovir as a positive control to screen novel candidate antivirals, notes Hayman, but we were shocked to find that acyclovir was at least an order of magnitude less effective in the submerged model (in which HSV mainly infected keratinocytes) than in the ALI model (in which HSV mainly infected fibroblasts)

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Incubate the plate at 37oC for 10 minutes